(1) Kev Tshawb Fawb Kev Txhim Kho ntawm CHLOROGENIC ACID
1. Anti-inflammatory thiab antioxidant
Thaum tshav kub kub kev ntxhov siab muaj qhov cuam tshuam loj rau kev ua haujlwm ntawm cov nqaij qaib. Chlorogenic acid (CGA) muaj cov kab mob tua kab mob, tiv thaiv inflammatory thiab antioxidant teebmeem. Chen Fang et al. (2021) tau kawm txog qhov cuam tshuam ntawm kev noj zaub mov CGA ntxiv rau kev noj qab haus huv hauv plab hnyuv thiab cecal microflora muaj pes tsawg leeg ntawm cov menyuam qaib nyob rau hauv kev ntxhov siab kub ntxhov, thiab muab faib ua 100-hnub-hnub Hy-line xim av qaib rau hauv 4 pawg. Cov qaib hauv pawg tswj hwm (C) thiab pab pawg kev ntxhov siab kub (HS) tau noj zaub mov basal, thiab hens hauv HS ntxiv rau CGA300 thiab HS plus CGA600 pawg tau noj cov zaub mov basal ntxiv nrog 300 thiab 600mg / kg CGA rau 2 lub lis piam, raws li. Broilers hauv HS pawg, HS ntxiv rau CGA 300 pab pawg thiab HS ntxiv rau CGA 600 pab pawg tau raug rau 38 degree rau 4h, thaum pab pawg tswj tau tuav ntawm 25 degree. Cov txiaj ntsig tau pom tias kev noj zaub mov CGA tuaj yeem txo qis ntawm T-AOC thiab T-SOD kev ua ub no thiab nce ntawm IL-1 thiab TNF- tshwm sim los ntawm kev kub ntxhov. Kev noj zaub mov ntxiv ntawm 600mg / kg CGA tuaj yeem txhim kho cov kab mob uas muaj txiaj ntsig zoo, xws li: Cov txheeb ze ntawm cov kab mob muaj txiaj ntsig zoo xws li Rikenellaceae RC9_ plab_ pawg, Ruminococcaceae UCG-005 thiab Christensenellaceae R-7_Pab tuaj yeem txo cov kab mob uas muaj feem cuam tshuam, xws li Suttelia. Yog li ntawd, CGA tuaj yeem kho qhov mob kub ntxhov los ntawm kev tiv thaiv kev mob, ua kom muaj peev xwm antioxidant thiab tswj cov kab mob cecal microflora.
2. Inhibit cov kab mob thiab tswj txoj hnyuv ua haujlwm
-- Zhang Xu et al. (2020) Tshawb xyuas qhov cuam tshuam ntawm chlorogenic acid (CA) ntawm plab hnyuv noj qab haus huv, kev loj hlob kev ua haujlwm, oxidative stress, inflammatory teb thiab ntshav biochemical indices ntawm SPF qaib kis nrog Clostridium perfringens (CP). Cov txiaj ntsig tau pom tias chlorogenic acid tuaj yeem txhim kho txoj kev loj hlob zoo, inhibit qhov kev puas tsuaj ntawm cov hnyuv me, txhim kho kev muaj peev xwm antioxidant, inhibit qhov kev puas tsuaj ntawm ileum mucosa thiab nruj kev sib txuas, inhibit inflammatory cytokines thiab txhim kho cov ntshav biochemical indexes. Zhuo Chunliu et al. (2020) tau kawm tias tom qab lub plab hnyuv kab mob nrog EScherichia coli O78, qhov kev tuag ntawm cov menyuam qaib nce ntxiv, cov plab hnyuv ntxwm ua haujlwm tsis zoo, cecal microflora hloov pauv, thiab kev tiv thaiv tsis zoo. Txawm li cas los xij, chlorogenic acid supplementation tended nce cov ntsiab lus ntawm IgA thiab IgM nyob rau hauv cov ntshav ntawm qaib cov kab mob EScherichia coli O78, txo cov kev qhia ntawm il-1 , IL-2 thiab IFN- hauv cov ntshav, txhawb kev qhia. ntawm jejunum nruj junction protein noob, thiab nce tus txheeb ze abundance ntawm Firmicide nyob rau hauv cecum ntawm qaib nyob rau hauv chlorogenic acid pawg. Qhov txheeb ze ntawm Bacteroidetes thiab Proteobacteria txo qis. Cov txiaj ntsig tau pom tias stevia chlorogenic acid los ntawm inhibiting plab hnyuv proinflammatory cytokines noob qhia, ua kom lub zog ntawm lub cev tiv thaiv kab mob thiab kev tiv thaiv kab mob humoral, thiab los ntawm kev ua kom lub plab zom mov nruj hlwv protein noob qhia thiab optimization ntawm plab hnyuv microbial flora, yog li inhibiting plab hnyuv o, txhim kho. lub plab hnyuv muaj nuj nqi, txhim khu qe qaib tiv thaiv kab mob, tiv thaiv e. kab mob coli.
-- Yao Shanshan et al. (2020) tau tham txog kev tshem tawm cov tshuaj tiv thaiv ntawm qaib escherichia coli los ntawm chlorogenic acid los ntawm transcriptomic sequencing thev naus laus zis, thiab 1.25mg / mL chlorogenic acid tuaj yeem txo MIC ntawm levofloxacin rau cov tshuaj tiv thaiv rov qab los ntawm 16ug / mL rau 4-8 ug/ml. Nws tuaj yeem txo qhov kev ntxhov siab ntawm cov kab mob, inhibit qhov kev ua ntawm cov tshuaj tiv thaiv cov noob thiab inhibit qhov rov ua dua ntawm cov kab mob DNA, uas tuaj yeem tshem tawm levofloxacin tsis kam ntawm cov tshuaj tiv thaiv EScherichia coli rau qee qhov, thiab yog li ua lub luag haujlwm ntawm inhibition thiab tshem tawm cov tshuaj. tsis kam.
- huab tais Yexus Qiang (2020), cov txiaj ntsig tau pom tias chlorogenic acid tuaj yeem txo qis LPS ua rau lub plab hnyuv raug mob, thiab chlorogenic acid ntawm cov kab mob plab hnyuv kab mob thiab kev ua haujlwm ntawm kev hloov kho tuaj yeem siv kev tiv thaiv kab mob plab hnyuv. Kev ua haujlwm ntawm chlorogenic acid, lub hauv paus los ntawm txoj hnyuv plab hnyuv rau hauv cov qauv kev tshawb fawb hauv vitro, raws li kev tsim cov kab mob hauv lub cev ntawm cov kab mob hauv nruab nrog cev raug tsim los ntawm LPS, Ua ke nrog JAK signaling pathway inhibitors, cov txheej txheem ntawm chlorogenic acid tswj txoj hnyuv qia cell muaj nuj nqi tau tshawb pom. . Cov txiaj ntsig tau pom tias qhov concentration ntawm 1mM chlorogenic acid txo qis los ntawm 0.5mM mus rau 0.1 mM hauv duodenum tom qab plab zom mov, thiab caffeic acid thiab quinic acid tau tsim thaum lub sijhawm ua haujlwm no. Chlorogenic acid txhawb nqa qhov kev qhia ntawm JAK signaling txoj hauv kev lPS-induced plab hnyuv raug mob apoptosis theem. Chlorogenic acid plays lub luag haujlwm tiv thaiv kab mob hauv plab hnyuv epithelial los ntawm kev ua kom muaj cov kab mob hauv plab hnyuv los ntawm kev tswj hwm ntawm JAK signaling txoj hauv kev loj hlob ntawm cov hnyuv raug mob nas.
- Chlorogenic acid muaj cov nyhuv bacteriostatic rau Clostridium perfringens thiab Escherichia coli, tuaj yeem tshem tawm cov tshuaj tiv thaiv ntawm EScherichia coli, txo qis lPS-vim plab hnyuv raug mob ntawm EScherichia coli, thiab tswj txoj hnyuv ua haujlwm.
3. Tswj kev tiv thaiv kab mob thiab tshuaj tiv thaiv kab mob
-- Muhammad Abaidullah et al. (2021) tau kawm txog cov tshuaj tiv thaiv kab mob ntawm CGA tiv thaiv kab mob ntsws ntsws (IBV) hauv vivo thiab hauv vitro thawj zaug. Cov kev sim hauv vitro tau lees paub tias CGA txo qis tus txheeb ze mRNA qhia ntawm IBV-N hauv CEK hlwb. 400mg / kg CGA-H tuaj yeem txo qhov kev qhia theem ntawm IBV-N mRNA thiab txo qhov raug mob ntawm trachea thiab ntsws. Cov kev qhia theem ntawm IL-6, IL-1 , IL-12 thiab NF-κB mRNA hauv trachea tau txo qis, thaum cov lus qhia theem ntawm IL-22 thiab IL{{ 11}} tau nce. Cga-h kev kho mob tau nce qib kev qhia ntawm MDA5, MAVS, TLR7, MyD88, IRF7, IFN- thiab IFN- hauv trachea thiab ntsws. Cga-h ua rau muaj kev loj hlob ntawm CD3 ntxiv , CD4 ntxiv thiab CD4 ntxiv / CD8 ntxiv , thiab ua rau cov ntshav nce ntau ntxiv IgA, IgG thiab IgM. Nyob rau hauv xaus, siab concentration ntawm CGA yog ib tug muaj zog tiv thaiv IBV compound, uas muaj peev xwm tswj innate tiv thaiv kab mob los ntawm MDA5, TLR7 thiab NF-κB txoj kev taw qhia, thiab induce cellular thiab humoral tiv thaiv kab mob nyob rau hauv CHICKENS kis IBV.
-- Li Yinju et al. (2021) Txhawm rau tshawb xyuas cov teebmeem tiv thaiv ntawm baicalin thiab chlorogenic acid ntawm cov lus teb inflammatory tshwm sim los ntawm IBDV kab mob hauv qaib embryos. Nqaij qaib embryo thaum muaj hnub nyoog 9 hnub tau muab faib ua 3 pawg thiab kho nrog ntau qhov sib txawv ntawm baicalin thiab chlorogenic acid thiab tsis muaj kev kho mob raws li kev tswj hwm. 48 teev tom qab hatching, txhua pab pawg tau inoculated nrog ib tug virulence. Kev ciaj sia ntawm cov embryos tau saib xyuas txhua hnub, thiab cov kab mob hauv lub siab thiab cov ntsiab lus ntawm cov kab mob inflammatory hauv lub siab tau kuaj pom los ntawm QRT-PCR. Thaum baicalin thiab chlorogenic acid concentrations yog 108 thiab 215mg / qe, ntsig txog, lawv pom muaj peev xwm tiv thaiv kab mob tseem ceeb, txo qis cov tshuaj histamine, cuam tshuam qhov ua kom nf-kb, thiab yog li inhibiting zus tau tej cov pro-inflammatory cytokines TNF- thiab il{ {11}}. Cov txiaj ntsig tau pom tias baicalin thiab chlorogenic acid muaj cov khoom tiv thaiv IBDV thiab pab tiv thaiv kab mob ntsig txog mob.
- Chlorogenic acid muaj cov tshuaj tua kab mob zoo rau cov kab mob bronchitis (IBV) thiab Bursa ntawm Fabricius virus (IBDV), feem ntau yog los ntawm inhibiting qhov kev qhia ntawm inflammatory yam, ua kom lub cev tiv thaiv kab mob, kom ua tiav cov txiaj ntsig ntawm kev tiv thaiv kab mob.
(2) APPLICATION PROSPECTS
Cov ntaub ntawv tshawb fawb tam sim no qhia tau tias chlorogenic acid muaj cov tshuaj tiv thaiv kab mob, tshuaj tua kab mob antioxidant, bacteriostatic, kev txhim kho plab hnyuv, tshuaj tiv thaiv kab mob thiab kev tiv thaiv kab mob, thiab tuaj yeem txo qhov kev pheej hmoo ntawm ntau yam kab mob ntawm cov hnyuv. Chlorogenic acid yog lub ntuj, ntsuab thiab nyab xeeb zaub mov additive thiab pub additive, uas tuaj yeem hloov cov tshuaj lom neeg kev loj hlob (tshuaj tua kab mob) thiab kev tiv thaiv kab mob, thiab muaj daim ntawv thov dav dav.
