Ntawm ntau yam teeb meem hauv kev loj hlob ntawm cov qoob loo, cov nroj tsuag tshwj xeeb yog kev puas tsuaj thiab ua rau cov qoob loo poob ntau dua li kev sib xyaw ua ke los ntawm cov kab mob thiab kab tsuag thoob ntiaj teb. Yog tias tsis tswj cov nroj tsuag, kwv yees li 33 feem pua -53 feem pua ntawm cov qoob loo yuav raug puas tsuaj. Kev lig kev cai, tshuaj tua kab mob tau siv los tshem tawm cov nroj tsuag hauv kev cog qoob loo loj ntawm Suav tshuaj ntsuab. Txawm li cas los xij, thaum cov tshuaj tua kab mob cuam tshuam rau kev loj hlob thiab kev loj hlob ntawm cov nroj tsuag, lawv kuj muaj ntau dua los yog tsawg dua kev cuam tshuam rau Suav tshuaj ntsuab lawv tus kheej, uas yuav muaj feem cuam tshuam rau cov txiaj ntsig, zoo thiab kev kho mob ntawm Suav tshuaj ntsuab. Tsis tas li ntawd, kev siv tshuaj tua kab mob ntau ntau tuaj yeem ua rau cov nroj tsuag tuaj yeem tiv taus, ua rau cov nroj tsuag tom qab tswj tau nyuaj dua. Chemosensitisation yog hais txog cov txiaj ntsig zoo thiab tsis zoo ntawm cov metabolites theem nrab ntawm cov nroj tsuag, kab mob, fungi thiab algae ntawm kev loj hlob thiab kev loj hlob ntawm cov kab mob ua liaj ua teb thiab ntuj tsim, uas yog hu ua chemosensitised tshuaj. Kev siv cov tshuaj chemosensitive raws li cov tshuaj tua kab mob los ntawm cov nroj tsuag tau dhau los ua cov ntsiab lus kub ntawm kev tshawb fawb, vim lawv tsis muaj qhov seem lossis tshuaj lom.
Mugwort yog nplooj qhuav ntawm Artemisia argyi Levl. thiab Vant. ntawm tsev neeg Asteraceae, uas muaj cov nyhuv ua kom sov lub cev ntas thiab nres los ntshav, dispersing txias thiab txo qhov mob; nws yog siv sab nraud los dispel dampness thiab txo qhov khaus. Raws li thaum ntxov Suav poetic vaj lug kub, nws tau sau tseg tias moxa nplooj tau siv los ua kom hluav taws, thiab thawj phau ntawv kho mob hauv Suav teb, Yellow Emperor's Classic of Internal Medicine, tau sau txog kev siv moxa nplooj rau kev kho mob moxibustion. Cov kws tshuaj niaj hnub tau pom tias cov nplooj mugwort muaj ntau yam tshuaj siv tshuaj xws li tshuaj tua kab mob, tshuaj tua kab mob, tshuaj tua kab mob, haemostatic, antitumour, hepatoprotective thiab choleretic, antioxidant, hnoos suppressant, analgesic thiab anti-inflammatory, hypoglycemic thiab immunomodulatory, nrog phenolic acids, flavonoids thiab nws cov ntsiab lus tseem ceeb. cov tshuaj pharmacological. Txawm li cas los xij, pab pawg tau pom nyob rau hauv thaj chaw uas mugwort muaj kev sib tw muaj zog rau ecological niches thiab yog lub zej zog tseem ceeb nrog ob peb cov nroj tsuag nyob ib puag ncig thiab tsis tshua muaj biodiversity, qhia tias mugwort yuav muaj kev cuam tshuam ntau dua kev lom zem thiab ecological nyhuv. Lub caij no, nyob rau hauv ib qho kev sim yav dhau los ntawm kev cog qoob loo ntawm Suav tshuaj ntsuab, mugwort hmoov tau tsim los siv los ua chiv rau chrysanthemum, echinacea thiab atractylodes, thiab nws tau pom los ntawm lub caij nyoog tias qhov nruab nrab thiab siab mugwort hmoov kho muaj qhov cuam tshuam tseem ceeb ntawm cov nroj tsuag. hom, naj npawb thiab biomass nyob rau hauv lub teb ntawm chrysanthemum, echinacea thiab atractylodes piv rau kev tswj dawb paug. Cov txiaj ntsig tau hais tias cov nplooj mugwort tuaj yeem muaj cov txiaj ntsig tseem ceeb ntawm cov tshuaj tua kab mob thiab muaj peev xwm tsim tau los ua cov tshuaj tua kab mob los ntawm cov nroj tsuag, yog li nws cov tshuaj tua kab mob tau raug tshuaj xyuas thiab cov khoom siv hauv paus thiab cov txheej txheem ntawm kev txiav txim tau tshawb xyuas.
Thawj thawj zaug, pab pawg tau pom tias aqueous extracts ntawm mugwort nplooj muaj zog inhibitory kev tiv thaiv tawm tsam monocotyledonous weeds Setaria viridis thiab Echinochloa crusgalli, thiab dicotyledonous weeds Portulaca oleracea thiab Amaranthus retroflexus. Siv cov tshuaj tua kab mob los ua kev qhia thiab ua ke nrog LC-MS txhawm rau ua kom muaj kev sib cais, phenolic acid compound isochlorogenic acid A tau pom tias yog cov tshuaj tseem ceeb hauv cov tshuaj mugwort thiab tau tshawb pom hauv cov av loj hlob ntawm mugwort, qhia tias mugwort. tuaj yeem cuam tshuam cov nroj tsuag loj hlob los ntawm kev tso tawm ntawm isochlorogenic acid A rau hauv ib puag ncig. Nyob rau tib lub sijhawm, qhov dav dav ntawm cov nroj tsuag inhibiting kev ua ntawm isochlorogenic A tau lees paub los ntawm inhibition ntawm kev loj hlob ntawm cov nroj tsuag nyob rau rau lub tsev neeg sib txawv, qhia tias isochlorogenic A tuaj yeem siv los ua tshuaj ntsuab ntsuab thiab tseem tuaj yeem muab zaub mov rau kev xav thiab kev tshoov siab rau cov tshiab. herbicides raws li ib tug pioneer compound.
Fig. 1 Kev ua-qhia kev sib cais ntawm chemosensitive active tshuaj los ntawm aqueous extracts ntawm mugwort nplooj
Daim duab 2 Kev ntsuam xyuas ntawm qhov dav-spectrum herbicide kev ua ntawm isochlorogenic acid A
Kev tshuaj xyuas lub cev tau pom tias isochlorogenic acid A kev kho mob ua rau cov nplooj ntoo kom tawg, daj thiab tuag. Lub teeb microscopy thiab kis tau tus mob electron microscopy qhia tias isochlorogenic acid A ua rau nplooj epidermal cell shrinkage, enlarged nplooj kab mob vascular sheath hlwb thiab txo cov chlorophyll sab hauv; cell membrane yaj thiab detached los ntawm lub cell phab ntsa thiab organelles disintegrated.
Pab neeg tshawb fawb tau siv RNA-seq los piav qhia cov kev hloov pauv hauv cov ntaub ntawv sau tseg ntawm cov nroj tsuag tom qab isochlorogenic acid A kho. 716 noob tau qhia txawv, ntawm uas 439 tau nce- tswj thiab 277 down-regulated. "KEGG enrichment tsom xam qhia tias inhibition ntawm "diterpenoid biosynthesis" txoj kev yog ib qho tseem ceeb mechanism ntawm kev txiav txim rau cov herbicidal kev ua si ntawm isochlorogenic acid A, feem ntau inhibiting nroj gibberellins thiab phytoplankton.
