Cov khoom siv ua haujlwm ntawm cov tshuaj suav tshuaj ntsuab yog cov khoom siv hauv paus rau Suav tshuaj kom ua haujlwm tau zoo, thiab nws tseem yog lub hauv paus ntawm nws cov kev tswj kom zoo. Pharmacological kev tshawb fawb nyob rau hauv xyoo tas los no tau qhia tias Gynostemma saponin yog lub ntsiab tshuaj tivthaiv ntawm Gynostemma pentaphyllum, nws cov qauv tshuaj lom neeg yog dammarane-type tetracyclic triterpenoid, thiab muaj ntau yam tshuaj muaj txiaj ntsig: 1. Nws tuaj yeem txhawb kev pw tsaug zog thiab kev ntxhov siab; 2. Nws tuaj yeem txo cov ntshav qab zib thiab ntshav rog; 3. Nws kuj ua lub luag haujlwm tseem ceeb hauv kev kho thiab tiv thaiv kab mob qog noj ntshav thiab qee yam kab mob ntev. Ntawm qhov tod tes, Gynostemma pentaphyllum extracts yog nplua nuj nyob rau hauv flavonols xws li kaempferol thiab quercetin, uas kuj muaj cov teebmeem zoo hauv inhibiting o thiab inhibiting kev loj hlob ntawm cov qog nqaij hlav cancer. Qhov no qhia tau hais tias Gynostemma Gynostemma saponins thiab flavonols nyob rau hauv Gynostemma pentaphyllum yog lub luag haujlwm ntawm Gynostemma pentaphyllum.
Txoj kev tshawb no tau ua qhov kev tshuaj ntsuam genome-wide ntawm R2R3-MYB tsev neeg ntawm Gynostemma pentaphyllum txhawm rau txheeb xyuas MYBs tseem ceeb koom nrog hauv kev tswj hwm ntawm Gynostemma saponins thiab flavonols hauv Gynostemma nplooj. Tag nrho ntawm 87 R2R3-GpMYB noob tau txheeb xyuas hauv Gynostemma Gynostemma genome, uas tuaj yeem muab faib ua 32 pawg. Feem ntau cov noob uas muaj cov haujlwm zoo sib xws hauv tib pab pawg muaj cov qauv exon-intron zoo sib xws thiab cov qauv motif. Cov R2R3-GpMYBs no tau faib tsis sib npaug ntawm 11 chromosomes. Txoj kev tshawb no pom tias qhov nthuav dav ntawm R2R3-GpMYB tsev neeg hauv Gynostemma genome feem ntau yog tshwm sim los ntawm segmental duplication. Kev tshuaj xyuas Ka/Ks tau qhia tias feem ntau ntawm cov thev R2R3-GpMYB noob tau raug xaiv kom huv.
Txhawm rau txheeb xyuas qhov twg R2R3-GpMYB noob tuaj yeem koom nrog hauv kev tswj hwm ntawm Gynostemma saponin thiab flavonol biosynthesis hauv Gynostemma nplooj, peb pom tias muaj peb R2R3-GpMYB noob (GpMYB60, GpMYB80 thiab GpMYB81) tau pom. nyob rau hauv cov kev tswj hwm network modules muaj feem xyuam rau biosynthesis ntawm Gynostemma saponins thiab flavonols, thiab tej zaum yuav muaj kev koom tes nyob rau hauv regulating lub biosynthesis ntawm Gynostemma saponins thiab flavonols. qRT-PCR cov txiaj ntsig tau pom tias GpMYB81 tau koom nrog Gynostemma saponin biosynthesis pathway noob GpFPS1, GpSS1, GpSE2 thiab GpOSC1 thiab flavonol biosynthesis pathway noob Gp4CL, GpCHS, GpCHI, GpF3H thiab. Tsis tas li ntawd, los ntawm cov poov xab ib-hybrid thiab dual luciferase thwmsim, nws tau txheeb xyuas tias GpMYB81 tuaj yeem khi rau cov neeg txhawb nqa ntawm Gynostemma saponin biosynthesis pathway gene GpFPS1 thiab flavonol biosynthesis pathway gene GpFLS thiab qhib lawv cov kev qhia.
Daim duab 1 Kev tshuaj xyuas ua ntej ntawm cov txheej txheem tswj hwm ntawm GpMYB81
Hauv kev xaus, cov txiaj ntsig ntawm txoj kev tshawb no muab cov ntaub ntawv tshiab rau kev tshuaj xyuas lub luag haujlwm ntawm R2R3-GpMYB noob hauv kev tswj hwm cov khoom siv ntawm Gynostemma pentaphyllum thiab ntxiv kev soj ntsuam kev ua haujlwm ntawm R2R3-GpMYB noob tsev neeg, thiab muab lub hauv paus tseem ceeb theoretical rau kev tswj hwm ntawm biosynthesis ntawm kev ua haujlwm ntawm Gynostemma pentaphyllum.
