Eugenol (4-Allyl-2-methoxyphenol) yog ib chav kawm tseem ceeb ntawm benzene nplhaib / phenylpropionic acid volatiles dav nyob rau hauv cov nroj tsuag paj thiab cov txiv hmab txiv ntoo laus, thiab muaj ntau yam teebmeem lom thiab kev noj qab haus huv. Eugenol tuaj yeem nyiam lom pollination thiab kis cov noob, tiv thaiv herbivores, thiab tseem tuaj yeem siv los ua tus neeg sawv cev tiv thaiv kom tsis txhob cuam tshuam cov kab mob parasitic xws li cov kab mob; Eugenol kuj tseem muaj cov tshuaj tua kab mob antioxidant, tshuaj tua kab mob, tshuaj tua kab thiab tshuaj tsw qab, thiab tau txais kev saib xyuas los ntawm cov kws tshawb fawb. . Nyob rau tib lub sijhawm, eugenol yog ib qho tseem ceeb ntawm cov txiv pos nphuab txiv pos nphuab, tab sis nws cov ntsiab lus hauv octoploid cultivated strawberry txiv hmab txiv ntoo yog qhov qis dua li hauv diploid qus-hom strawberry. Txog niaj hnub no, txoj kev biosynthetic ntawm eugenol tau nkag siab ntau hauv cov nroj tsuag, thiab feem ntau ntawm cov phenylpropanoid txoj kev tswj hwm (xws li R2R3-MYB transcription yam FaMYB10 thiab FaEOBII, DNA-binding zinc ntiv tes transcription factor FaDOF2) tau validated nyob rau hauv strawberries Lub ripening theem plays lub luag hauj lwm tom qab nyob rau hauv cov txiv hmab txiv ntoo txoj kev loj hlob, thaum eugenol cov ntsiab lus yog siab dua nyob rau hauv strawberry txiv hmab txiv ntoo nyob rau hauv lub loj ntsuab txiv hmab txiv ntoo theem, tab sis nws tsis paub meej li cas eugenol tsub zuj zuj yog transcriptionally tswj nyob rau theem no.
Hauv strawberries, eugenol synthase-encoding genes FaEGS1 thiab FaEGS2 thiab cinnamyl cawv dehydrogenase-encoding gene FaCAD1 yog qhov tseem ceeb rau eugenol ntau lawm. FaEGS1 thiab FaEGS2 tau qhia tshwj xeeb hauv strawberry ntsuab txiv hmab txiv ntoo thiab txiv hmab txiv ntoo siav, feem. Nyob rau hauv txoj kev tshawb no, txhawm rau txheeb xyuas cov xwm txheej hloov pauv uas cuam tshuam nrog kev tswj hwm ntawm FaEGS1, kev tshuaj ntsuam poov xab ib leeg-hybrid tau ua los ntawm kev siv FaEGS1 tus txhawb nqa los ua kab nuv ntses, thiab R2R3-MYB transcription factor FaMYB63 tau kawm ntxiv hauv kev tshuaj ntsuam. tshwm sim. FaMYB63 tau nyob rau hauv lub nucleus, thiab RT-qPCR tsom xam pom tias FaMYB63 qhia yog cov ntaub so ntswg- thiab theem tshwj xeeb, raws li eugenol cov ntsiab lus thaum lub sij hawm strawberry txiv hmab txiv ntoo txoj kev loj hlob, thiab yog inhibited los ntawm abscisic acid (ABA) thiab qhib los ntawm auxin (IAA). FaMYB63 overexpression thiab RNAi-mediated silencing ua rau muaj kev hloov pauv tseem ceeb hauv cov ntawv teev lus ntawm eugenol synthesis genes FaEGS1, FaEGS2 thiab FaCAD1, uas nyob rau hauv lem cuam tshuam eugenol tsub zuj zuj. EMSA, poov xab monohybrid, GUS kev ua ub no thiab dual luciferase kev soj ntsuam kev ua ub no qhia tau hais tias cov transcription ntawm FaEOBII thiab FaMYB10 kuj tau tswj los ntawm FaMYB63, tab sis tsis vice versa. Ua ke, cov txiaj ntsig no muab kev nkag siab tshiab rau hauv kev tswj hwm kev tswj hwm ntawm strawberry eugenol synthesis txoj hauv kev.
