Tsis ntev los no, Peng Cheng pab neeg los ntawm Lub Xeev Lub Chaw Haujlwm Tseem Ceeb ntawm Southwest Suav Tshuaj Cov Chaw Pabcuam ntawm Chengdu University of Traditional suav tshuaj siv cov tshuaj uas tsis yog hom phiaj GC-MS thiab UHPLC / Q-Orbitrap MS ua ke nrog ntau qhov sib txawv thiab tsis sib xws los tshuaj xyuas cov metabolic profiles ntawm cov roj tsis haum. thiab methanol rho tawm los ntawm ntau qhov chaw ntawm Chuanxiong Studies tau ua thiab cov kev ua antioxidant, vasodilatory kev ua si thiab anticoagulant kev ua ntawm extracts los ntawm ntau qhov chaw tau soj ntsuam. Txoj kev tshawb no muab cov ntaub ntawv tseem ceeb rau kev tshawb fawb thiab kev loj hlob ntawm cov khoom siv tshuaj suav tshuaj hauv Suav teb txhawm rau tshawb nrhiav cov peev txheej siv cov khoom siv pov tseg ntawm cov tshuaj suav tshuaj Chuanxiong, kom paub tias "ua kom zoo tshaj plaws siv cov ntaub ntawv thiab tig pov tseg rau hauv cov khoom muaj nqis" .
Cov tshuaj ib txwm siv ntawm chuanxiong (Ligusticum chuanxiong Hort.) yog cov rhizomes, thaum lub stems, nplooj thiab fibrous cov hauv paus hniav uas nplua nuj nyob rau hauv cov kev pab cuam yog "tsis yog tshuaj", uas yog ib txwm muab pov tseg. Pab neeg tshawb fawb kev tshawb fawb yav dhau los pom tias cov stems thiab nplooj ntawm Chuanxiong muaj ntau tus phthalides nrog cov qauv tshiab uas ua rau kom cov hlab ntsha so. Raws li kev tshawb fawb txog kev sib raug zoo ntawm cov qauv-kev sib raug zoo, pab pawg siv electrophysiology thiab calcium imaging thwmsim, ua ke nrog homology qauv thiab molecular docking tshuab, xaiv peb Cav1.2 antagonist lead compounds los ntawm lub tsev qiv ntawv compound. Qhov chaw tshawb fawb loj heev rau kev txhim kho cov tshuaj tshiab (Bioorg. Chem., 2021, 107, 104523, nyem los nyeem kom paub meej). Txawm li cas los xij, kev faib tawm ntawm cov khoom siv bioactive hauv Chuanxiong tseem tsis paub meej, thiab ntau hom phthalide feem ntau yuav tsum tau tshawb nrhiav ntxiv. Nyob rau hauv txoj kev tshawb no, 75 tebchaw nyob rau hauv lub volatile roj ntawm txawv qhov chaw (kab nplooj, fibrous cov hauv paus hniav, rhizomes) ntawm Chuanxiong tau characterized los ntawm GC-MS. Siv UHPLC/Q-Orbitrap MS technology, tag nrho ntawm 243 tebchaw, suav nrog 95 phthalide tebchaw, tau txheeb xyuas los ntawm cov tshuaj methanol los ntawm ntau qhov chaw. Vim qhov pom tseeb isomerism ntawm phthalide tebchaw, cov txiaj ntsig zoo raws li kev sib piv cov ntaub ntawv ib txwm tsis zoo tagnrho. Pab neeg tshawb fawb tau txheeb xyuas cov khoom phthalide raws li lub chaw soj nstuam tus kheej tsim cov ntaub ntawv, kev tshawb fawb cov ntaub ntawv thiab kev tshuaj xyuas ntawm txoj kev tawg, suav nrog 14 lub peev xwm tshiab. Feem ntau, cov tshuaj muaj pes tsawg leeg ntawm qhov sib txawv ntawm Chuanxiong zoo ib yam, thiab phthalide yog lub ntsiab ntawm txhua feem. Nws yog ib nqi sau cia hais tias nyob rau hauv lub methanol extract, cov ntsiab lus ntawm monophthalide yog siab dua nyob rau hauv rhizomes thiab fibrous cov hauv paus hniav, thaum phthalide dimers los yog phthalide trimers tau ntau faib nyob rau hauv stems thiab nplooj (Fig. 3). Cov txiaj ntsig ntawm PCA thiab HCA tsom xam tau faib cov chaw sib txawv rau hauv ntau pawg (Fig. 4). Cov metabolites sib txawv tau raug tshuaj xyuas los ntawm tus nqi VIP, tus nqi FC, thiab p tus nqi thiab pom tau tias siv daim duab tshav kub (Daim duab 5). Cov txiaj ntsig tau pom tias cov tshuaj muaj pes tsawg leeg ntawm qhov sib txawv ntawm Chuanxiong txawv. Hauv kev soj ntsuam kev ua si ntawm ntau qhov chaw, stems thiab nplooj muaj qhov zoo tshaj plaws antioxidant kev ua haujlwm, cov hauv paus hniav fibrous muaj qhov muaj zog vasodilatory kev ua si, thiab rhizomes muaj qhov tseem ceeb tshaj plaws anticoagulant kev ua haujlwm (Fig. 6). Qhov no yog qhov tshwm sim ntawm qhov sib txawv ntawm cov metabolites hauv ntau qhov chaw thiab tseem qhia txog "ib lub cev, ntau lub hom phiaj" xwm txheej ntawm cov tshuaj suav tshuaj. Feem ntau, cov khoom tsis yog tshuaj ntawm Chuanxiong muaj ntau cov khoom siv lom neeg, thiab cov qauv kev sib txawv ntawm phthalide yog nplua nuj, qhia tau zoo heev hauv kev tshawb fawb ntawm lwm cov peev txheej thiab kev tsim cov tshuaj tshiab.
Kev faib tawm ntawm cov khoom siv tshuaj hauv ntau qhov chaw ntawm Chuanxiong. Volatile Roj (a); Methanol Extract (b)
Cov qhab nia PCA thiab HCA daim duab ntawm cov roj tsis hloov pauv thiab cov tshuaj methanol rho tawm ntawm ntau qhov chaw ntawm Chuanxiong. PCA daim ntawv qhia ntawm cov roj tsis hloov pauv (a); PCA daim ntawv qhia ntawm methanol extract (b); HCA daim ntawv qhia ntawm cov roj volatile (c); HCA daim ntawv qhia ntawm methanol extract (d)
Daim ntawv qhia tshav kub ntawm cov metabolites sib txawv hauv cov roj tsis hloov pauv (a) thiab methanol extract (b) ntawm qhov sib txawv ntawm Chuanxiong
Pearson correlation matrix ntawm IC50 qhov tseem ceeb ntawm cov dawb radical scavenging tus nqi ntawm DPPH thiab ABTS thiab cov ntsiab lus ntawm ntau yam khoom (a); vasodilatory kev ua ub no ntawm ntau qhov chaw ntawm Chuanxiong (b); Cov teebmeem ntawm qhov sib txawv ntawm Chuanxiong ntawm APTT, PT thiab TT coagulation lub sij hawm hauv nas (ce)
Hauv txoj kev tshawb no, Chuanxiong, cov khoom siv tshuaj kho mob tiag tiag los ntawm Sichuan, tau siv los ua piv txwv, thiab ntau txoj hauv kev tau siv los tshuaj xyuas thiab sib piv qhov sib txawv ntawm cov khoom siv tshuaj lom neeg thiab kev ua haujlwm lom neeg ntawm ntau qhov chaw. Cov khoom muaj nqis", muab cov ntaub ntawv tseem ceeb rau kev tshawb fawb thiab kev loj hlob ntawm cov tshuaj suav tshuaj suav tshuaj. Qhov kev tshawb pom ntawm tus nqi ntawm cov tshuaj suav tshuaj pov tseg yuav txhawb kev hloov pauv ntawm kuv lub teb chaws cov khoom siv tshuaj suav tshuaj hauv kev lag luam mus rau kev txuag nyiaj thiab ib puag ncig- tus phooj ywg ncig kev lag luam kev lag luam qauv.
